Thursday 12 December 2013

How To Make Use Of The Dissolved Oxygen Test Kit

Posted by Unknown at 14:13
By Cornelia White


It is actually necessary for individuals to use a quality dissolved oxygen test kit. This is for the sake of determining what the health of an aquatic ecosystem is. This should also help in evaluating productivity and pollution in areas like lakes, streams, and rivers. Here are the important steps that people should take when they want to use this item.

First of all, prepare the BOD bottle. One will need to fill this up with a sample H2O. Simply dip the said bottle on the aquatic ecosystem one plans on testing. Wait for the bottle to fill up while ensuring that there are no bubbles coming inside. Tilt the bottle from side to side so that one can let the bubbles escape.

The next thing to prepare is the manganese sulfate. Mix this to the acquired sample. To make the process easier, one just needs to utilize a calibrated pipette. Insert the calibrated pipette into the surface of the said sample. Then, squeeze it slowly. Prevent air bubbles from getting inside as well.

It is also a given to pay close attention to the alkali-iodide-azide reagent. One will have to use this as this will be added to one's sample. Since the person is going to add this alkali-iodide-azide reagent into one's sample, one has to make use of the pipette as well. There should be less difficulties in adding this reagent into one's sample.

Stopper this bottle as carefully as possible. Also, do not allow any air bubbles to get inside. To check up on this, the bottle should be turned upside down. Do this a few times to easily mix the content. No bubbles should be inside the sample since having bubbles there means one has to discard this sample and start again from scratch.

The person should also remember to add the sulfuric acid. Like the other elements one has added beforehand, the sulfuric acid can be added with the use of a pipette. However, one should just tip this pipette above the surface instead of simply inserting it below the surface of the H2O. Put a stopper on the bottle and turn it over again, mixing the contents.

There should be an Erlenmeyer flask prepared beforehand too. The person will then have to place 201ml of the sample solution into the Erlenmeyer flask. Make sure to get the right amount for this to get better results. For the person to get the right amount for the sample solution, utilize a graduated cylinder. After that, add sodium thiosulfate with the pipette to change this into a pale straw color.

This time, one should then add starch solution into the sample. The starch solution should be in the amount of two milliliter. After adding the said solution, this sample should then turn to blue. Continue adding more sodium thiosulfate into the said solution until it becomes a clear color.

Do not forget to record how many sodium thiosulfate one has added to the solution. With this, one must be able to calculate the element content of the said sample. Since the person is going to use this dissolved oxygen test kit, assume that every sodium thiosulfate equates to one ml per liter of dissolved oxygen.




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